By Raymond on Feb 28, 2009 in Lab | 0 Comments
Undergraduate Lab Tips on maintaining fruitflies by yourself! Recipe for flies: Yummy!!! Water 50L Agar 470g Sucrose 1580g Glucose 3160g Preservative 142g Doxycycline (antibiotics) 2.1g Amoxicillin (antibiotics) 4.16g sodium tartrate (it is an emulsifier used in food like jellies) 438g calcium chloride 36.3g maize powder 3885g Of course, you may change the amount according [...]
By Raymond on Feb 28, 2009 in Lab | 0 Comments
Undergraduate Lab at Tsinghua University Research Alzheimer’s disease, or AD We have transgenic flies which express human Aβ42 or its mutant forms, which can be directly driven in fly neurons using UAS-GAL4 gene switch. These flies have defects in olfactory learning, memory and lifespan. Amyloid deposits and neurodegeneration appear when they get old, [...]
By Raymond on Feb 27, 2009 in Lab | 3 Comments
Undergraduate Lab at Tsinghua University Lab apparatus Brain section Cross section of fly head (left), and the machine for slicing and embedding the sample (right). Brain section helps to reveal neurodegeneration in neurological diseases and protein spatial expression through immunohistochemistry. Genetic tools and fluorescent microscopy With the UAS-GAL4 system and other gene switches, we [...]
