By Raymond on Feb 28, 2009 in Lab | 0 Comments
Undergraduate Lab
Tips on maintaining fruitflies by yourself!
Recipe for flies: Yummy!!!
Water
50L
Agar
470g
Sucrose
1580g
Glucose
3160g
Preservative
142g
Doxycycline (antibiotics)
2.1g
Amoxicillin (antibiotics)
4.16g
sodium tartrate (it is an emulsifier used in food like jellies)
438g
calcium chloride
36.3g
maize powder
3885g
Of course, you may change the amount according to the proportion. You can use a great pot to “cook” the food, or just a beaker with a microwave oven.
This is the formula [...]
By Raymond on Feb 28, 2009 in Lab | 0 Comments
Undergraduate Lab at Tsinghua University
Research
Alzheimer’s disease, or AD
We have transgenic flies which express human Aβ42 or its mutant forms, which can be directly driven in fly neurons using UAS-GAL4 gene switch. These flies have defects in olfactory learning, memory and lifespan. Amyloid deposits and neurodegeneration appear when they get old, as revealed by immunostaining and [...]
By Raymond on Feb 27, 2009 in Lab | 1 Comment
Undergraduate Lab at Tsinghua University
Lab apparatus
Brain section
Cross section of fly head (left), and the machine for slicing and embedding the sample (right). Brain section helps to reveal neurodegeneration in neurological diseases and protein spatial expression through immunohistochemistry.
Genetic tools and fluorescent microscopy
With the UAS-GAL4 system and other gene switches, we can easily control, monitor and track [...]
